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KMID : 0545120130230081159
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 8 p.1159 ~ p.1166
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Orientia tsutsugamushi Infection Induces CD4+ T Cell Activation via Human Dendritic Cell Activity
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Chu Hyuk
Park Sung-Moo
Cheon In-Su
Park Mi-Yeoun
Shim Byoung-Shik
Gil Byoung-Cheol
Jeung Woon-Hee
Hwang Kyu-Jam
Song Ki-Duk
Hong Kee-Jong
Song Man-Ki
Jeong Hang-Jin
Han Seung-Hyun
Yun Cheol-Heui
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Abstract
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Orientia tsutsugamushi, a gram-negative bacterium, causes severe acute febrile illness in humans. Despite this danger, the route of infection, infectivity, and protective mechanisms of the host’s immune response to O. tsutsugamushi are unclear. Dendritic cells (DCs) are one of the most important cell types in bridging the innate and adaptive immune responses. In this study, we observed that O. tsutsugamushi infects and replicates in monocyte-derived DCs (MODCs). During infection and replication, the expressions of the cytokines IL-12 and TNF-α, as well as the co-stimulatory molecules CD80, CD83, CD86, and CD40, were increased in MODCs. When O. tsutsugamushi-treated MODCs were co-cultured with autologous CD4+ T cells, they enhanced production of IFN-γ, a major Th1 cytokine. Collectively, our results show that O. tsutsugamushi can replicate in MODCs and can simultaneously induce MODC maturation and increase proinflammatory cytokine levels in MODCs that subsequently activate CD4+ T cells.
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KEYWORD
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Orientia tsutsugamushi, Dendritic cells, T cell, Proinflammatory cytokines
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